Rabbit Anti-Interleukin 10 Receptor alpha (IL10RA, IL-10RA, IL-10R-A, CDw210a, HIL-10R, IL-10R1, IL10R)
Interleukin-10 (IL-10) is a cytokine whose main biological function is to suppress the immune response by induction of a signal(s) leading to inhibition of synthesis of a number of cytokines and their cellular receptors. Signal transduction is initiated upon formation of a ternary complex of IL-10 with two of its receptor chains, IL-10R1 and IL-10R2, expressed on the cell membrane. The affinity of IL-10R1 toward IL-10 is very high, which allowed determination of the crystal structure of IL-10 complexed with the extracellular/soluble domain of IL-10R1, while the affinity of IL-10R2 toward either IL-10 or IL-10/sIL-10R1 complex is quite low.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence amd Flow Cytometry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Flow Cytometry: 1:10-1:50 Immunofluorescence: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 147-175 in the central region of human Interleukin 10 Receptor alpha, UniProt Accession #Q13651.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Interleukin 10 Receptor alpha.
