Rat Anti-IL12A (Interleukin-12 Subunit alpha, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35)
Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-g and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL-12 share 70-60aa sequence identity in their p40 and p35 subunits, respectively.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to aa23-215 from mouse IL12A expressed in Sf21 cells (NP_032377).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse IL12A. Species crossreactivity: recombinant mouse IL-12 p35+p40 heterodimer, recombinant rat IL-12 p35 (50%). Does not react with recombinant human IL-12 p35, recombinant mouse IL-12 p40, IL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, recombinant feline IL-12 p35+p40 heterodimer.
