Mouse Anti-Interleukin 16 (LCF, prIL-16, HST19289)
Interleukin-16, also known as lymphocyte chemoattractant factor (LCF), is a chemotactic cytokine that stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils through binding to the CD4 receptor. IL-16 induces human T-lymphocyte expression of interleukin 2 receptor and upregurates major histocompatibility complex molecules on resting human T lymphocyte. It is able to render CD4+ T cells unresponsive to TCR/CD3 stimuli. Moreover, IL-16 inhibits the replication of T cell and monocyte tropic HIV strains in vitro.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Matched Pair
Capture: I8438-11H Dectection: I8438-11G
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human IL-16.
Form
Supplied as a lyophilized powder in 0.01M PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human IL-16. Does not cross react with human serum albumin, bovine serum albumin or other human cytokines or growth factors tested such as IL-1b, IL-8, IL-7, MCAF, TGF-beta 1, EGF, bFGF, GM-CSF, M-CSF and TNF-alpha.
