Technical Data

I8443-93
Clone Type
Polyclonal
Host
Sheep
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-Interleukin 34 (IL-34, C16orf77, chromosome 16 open reading frame 77, hypothetical protein MGC34647)

Interleukin-34, or IL-34 is a newly discovered interleukin. It was found in a massive screen of secreted proteins by Lin et al. at Five Prime Therapeutics, a company based on the Mission Bay campus of UCSF. IL-34 increases monocyte viablity and acts through its receptor colony-stimulating factor 1. The protein is 39kD in size and forms homodimers.

Applications
Suitable for ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mIL-34. ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse IL-34. The detection limit for rmIL-34 is approximately 0.5ng/well. Optimal dilution determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, NS0-derived, recombinant mouse IL-34, Asn21-Pro235, (rmIL-34)
Form
Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IL-34 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 25% cross-reactivity with rhIL-34.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Lin H, Lee E, Hestir K, Leo C, Huang M, Bosch E, Halenbeck R, Wu G, Zhou A, Behrens D, Hollenbaugh D, Linnemann T, Qin M, Wong J, Chu K, Doberstein SK, Williams LT. Discovery of a cytokine and its receptor by functional screening of the extracellular proteome.Science. 2008 May 9;320(5877):807-11. PMID: 18467591
USBio References
No references available
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