Mouse Anti-Involucrin (IVL)
Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585aa, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene,encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry,. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Immunohistochemistry (paraffin, frozen): 0.4-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human involucrin.
Form
Supplied as a powder lyophilized from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitue with 1ml sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IInvolucrin. Species Crossreactivity: porcine, canine.