Technical Data

I8447-27F
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
10K258
Grade
Affinity Purified
Applications
IC IHC WB
Crossreactivity
Ca Hu Po
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Involucrin (IVL)

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585aa, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene,encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry,. Other applications not tested.
Recommended Dilution
Western Blot: 2ug/ml Immunohistochemistry (paraffin, frozen): 0.4-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human involucrin.
Form
Supplied as a powder lyophilized from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitue with 1ml sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IInvolucrin. Species Crossreactivity: porcine, canine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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