Goat Anti-IRE2 (Serine/Threonine-protein kinase/Endoribonuclease IRE2, Inositol-requiring 2, hIRE2p, IRE1b, Ire1-beta, Endoplasmic Reticulum-to-nucleus Signaling 2, Serine/Threonine-protein Kinase, Endoribonuclease, ERN2)
ERN2 induces translational repression through 28S ribosomal RNA cleavage in response to endoplasmic reticulum (ER) stress. This pro-apoptotic appears to play no role in the unfolded-protein response, unlike closely related proteins. Overexpression of ERN2 activates both BiP and CHOP expression, and also leads to the development of programmed cell death. It has been suggested that Ern2 plays a role in multiple facets of the ER stress response in mammalian cells.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QKRLQSPSKQAQPLD, from ERN2, at the internal region of the protein (NP_150296.3).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes ERN2. Species sequence homology: Human.
