Technical Data

I8700-18C
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_003740
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-IRS-2, CT (Insulin Receptor Substrate 2, IRS2)

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human IRS2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human IRS2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Hagg,D.A., Int. J. Mol. Med. 21(6), 697-704 (2008). 2.Platanias,L.C., J. Biol. Chem. 271(1), 278-282 (1996). 3.Sun,X.J., Nature 377(6545), 173-177 (1995).
USBio References
No references available
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