Rabbit Anti-ISL1 (IISL LIM Homeobox 1, Insulin Gene Enhancer Protein ISL-1, Isl-1, ISLET1, Islet-1)
Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5ug/ml detected ISL-1 on 10ug K562 cell lysate. Immunohistochemistry (Paraffin): 1:300 dilution from a previous lot detected ISL-1 in mouse pancreas tissue. Antigen retrieval with citrate, pH 6.0 is recommeneded. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Linear peptide corresponding to human ISL-1, conjugated to KLH.|Species Sequence Homology: equine, bovine, sheep, rat and canine; 100%
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide
Purity
Purified by affinity chromatography.
Specificity
Recognizes human ISL-1 at ~39kD. Species Crossreactivity: mouse
