Technical Data

J0400-05D
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
7H31(C83A8)
Grade
Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
3714
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Jagged2 (JAG2, HJ2)

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like 1 (Dll1), Dll3 and Dll4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the gamma-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).

Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding Pro1212 of human Jagged2. (KLH).
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human Jagged2 protein at 150kD. Does not cross-react with Jagged1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1 Wilson, A. and Radtke, F.FEBS Lett. 580: 2860-2868. (2006) . 2 Hansson, E.M. et al. Semin. Cancer Biol. 14: 320-328. (2004). 3 Chiba, S. Stem Cells 24: 2437-2447. (2006). 4 Bland, C.E. et al. J. Biol. Chem. 278: 13607-13610. (2003). 5 Six, E. et al. Proc. Natl. Acad. Sci. USA 100: 7638-7643. (2003). 6 LaVoie, M.J. and Selkoe, D.J. J. Biol. Chem. 278: 34427-34437.(2003).
USBio References
No references available
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