Mouse Anti-JNK1 (c-Jun N-terminal Kinase 1, JNK1A2, JNK, JNK21B1/2, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1)
EC=2.7.11.24
The mitogen-activated protein kinase (MAP) kinase family includes ERK, JNK and p38 kinase. The pathway, regulated by (MAP) kinase family, involves a broad array of cellular responses that include survival, cell proliferation, and apoptosis. The JNK signal transduction pathway is activated in response to environmental stress and by engagement of several classes of cell surface receptors including cytokine receptors, serpentine receptors and receptor tyrosine kinases. Although JNK is located in both the cytoplasm and the nucleus of quiescent cells, activation of JNK is associated with accumulation of JNK in the nucleus. In the NGF (nerve growth factor)-differentiated PC-12 cells, activation of the ERK signaling pathway promotes cell survival but stimulation of the JNK and p38 signaling pathways contributes to cell death.
Applications
Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended Dilution
Immunofluorescence: 5-10ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant GST-fusion protein of JNK1
Form
Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide
Purity
Purified by Protein A chromatography
Specificity
Reacts with JNK1 in both transfected and endogenous cell lines on Western blots. This antibody may crossreact with JNK3 in an overexpressed cell lysate, but the cross reactivity to JNK2 has not been observed. This antibody identifies a 54kD band on Western blot analysis.|Reactivity is confirmed with A431 cell lysates.
