Technical Data

K0005-11A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG
Clone Number
2F11
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Kallikrein 8 (hK8, Neuropsin, NP, Ovasin, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, Serine protease 19, KLK8, NRPN, PRSS19, TADG14)

Kalllikrein-8 is a serine protease capable of degrading several proteins such as casein, fibrinogen, kininogen, fibronectin and callagen type IV. It also cleaves L1CAM in response to increased neural activity. It induces neurite outgrowth and fasciculation of cultured hippocampal neurons. It plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. It is involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. It is Inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor. It is a secreted protein that has 4 named isoforms produced by alternative splicing. Isoform 1 is predominantly expressed in the pancreas while isoform 2 is expressed in adult brain and hippocampus. Both forms are also found in fetal brain and placenta. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples.

Applications
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. �
Immunogen
Partial recombinant human Kallikrein-8 (97-2040) with a GST tag.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human Kallikrein 8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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