K0199-12X-APC
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
APCIsotype
IgG1Clone Number
0.N.354 (DE-K10)Grade
Affinity PurifiedApplications
IF IHC WBCrossreactivity
Ca Fe Hu RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
BSA Free
Mouse Anti-Keratin 10 (BSA & Azide Free) (APC)
Keratin 10 (CK 10) is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. Expression of CK 10 is related to maturation of malignant keratinocytes, being preferentially detected in more differentiated parts.
Applications: Immunofluorescence, Western Blotting, Immunohistochemistry.
Recommended Dilution
Western Blotting: 1ug/ml Immunohistochemistry: 2-4ug/ml. Frozen & formalin/paraffin. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Optimal dilutions to be determined by the researcher.
Note: Applications are based on unconjugated antibody.
Immunogen
Cytoskeletal preparation extracted from human ectocervical epithelium. Cellular Localization: Cytoplasmic.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Keratin 10 at 56.5kD. Species Crossreactivity: rat, canine and feline.
