Technical Data

K0199-24E
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
BA17
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I cytoskeletal 19, Keratin-19, MGC15366)

Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis.

Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 2-4ug/ml for 2 hrs at RT Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher.
Positive Control: Ls174T and HT29 Cells, Colon ca.
Cellular Localization: Cytoplasmic
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Detergent soluble extract of human mammary epithelium.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes human Keratin 19.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bartek J, et al. (1985) J Cell Sci, 75:17-33.|2. Moll R, et al. (1982) Cell, 31:11-24.|3. Tseng S C G, et al. (1982) Cell, 30:361-72.|4. Bartek J, et al. (1986) Histochem J, 18:565-575.|5. Bartek J et al. (1985) Int J Cancer, 36:299-306.|6. Bartek J et al. (1986) Eur J Cancer Clin Oncol, 22:1441-52.|7. Bartkova J, et al. (1987) Tumor Biol, 8:45-56.|Suggested References:|1. Hazelbag HM; van den Broek LJ; van Dorst EB; Offerhaus GJ; Fleuren GJ; Hogendoorn PC. Immunostaining of chain-specific keratins on formalin-fixed, paraffin-embedded tissues: a|comparison of various antigen retrieval systems using microwave heating and proteolytic pretreatments.|Journal of Histochemistry and Cytochemistry, 1995, 43(4):429-37.|2. Narisawa Y; Hashimoto K; Kohda H. Immunohistochemical demonstration of keratin 19 expression in isolated human hair follicles. Journal of Investigative Dermatology, 1994, 103(2):191-5.|3. Akasofu M; Kawahara E; Kurumaya H; Nakanishi I. Immunohistochemical detection of breast specific antigens and cytokeratins in metastatic breast carcinoma in the liver. Acta Pathologica Japonica, 1993, 43(12):736-44.|4. Klijanienko J; el-Naggar A; De Braud F; Micheau C; Janot F; Luboinski B; Gentile A; Russo A; Cvitkovic E. Keratins 6, 13 and 19. Differential expression in squamous cell carcinoma of the head and neck. Analytical and Quantitative Cytology and Histology, 1993, 15(5):335-40.|5. Nasser IA; Lee AK; Bosari S; Saganich R; Heatley G; Silverman ML. Occult axillary lymph node metastases in "node-negative" breast carcinoma. Human Pathology, 1993, 24(9):950-7.|6. Su L; Morgan PR; Thomas JA; Lane EB. Expression of keratin 14 and 19 mRNA and protein|in normal oral epithelia, hairy leukoplakia, tongue biting and white sponge nevus. Journal of Oral Pathology and Medicine, 1993, 22(4):183-9.|7. Coltrera MD; Zarbo RJ; Sakr WA; Gown AM. Markers for dysplasia of the upper aerodigestive|tract. Suprabasal expression of PCNA, p53, and CK19 in alcohol-fixed, embedded tissue. American|Journal of Pathology, 1992, 141(4):817-25.|8. Mittal KR; Demopoulos RI; Goswami S. Patterns of keratin 19 expression in normal, metaplastic, condylomatous, atrophic, dysplastic, and malignant cervical squamous epithelium. American|Journal of Clinical Pathology, 1992, 98(4):419-23.|9. Nomoto M; Uchikosi Y; Kajikazawa N; Tanaka Y; Asakura H. Appearance of hepatocytelike cells in|the interlobular bile ducts of human liver in various liver disease states. Hepatology, 1992, 16:1199-205.|10. Carmichael RP; McCulloch CA; Zarb GA. Immunohistochemical localization and quantification|of desmoplakins I & II and keratins 1 and 19 in plastic-embedded sections of human gingiva. Journal|of Histochemistry and Cytochemistry, 1991, 39(4):519-28.|11. Bader BL; Franke WW. Cell type-specific and efficient synthesis of human cytokeratin 19 in|transgenic mice. Differentiation, 1990, 45:109-18.|12. Bartek J; Bartkova J; Taylor-Papadimitriou J. Keratin 19 expression in the adult and developing|human mammary gland. Histochemical Journal, 1990, 22(10):537-44.|13. Leoncini P; Petracca R; Ruggiero P; Cintorino M; Syrjanen S; Mantyjarvi R; Syrjanen K.|Expression of cytokeratin No. 19 polypeptide in genital papillomavirus lesions. Gynecologic and|Obstetric Investigation, 1990, 29(1):59-66.|14. Stosiek P; Kasper M; Karsten U. Expression of cytokeratin 19 during human liver organogenesis.|Liver, 1990, 10(1):59-63.|15. Van Eyken P; Sciot R; Callea F; Ramaekers F; Schaart G; Desmet VJ. A cytokeratinimmunohistochemical|study of hepatoblastoma. Human Pathology, 1990, 21(3):302-8.|16. Van Eyken P; Sciot R; Callea F; Ramaekers F; Schaart G; Desmet VJ. A cytokeratinimmunohistochemical|study of hepatoblastoma. Human Pathology, 1990, 21(3):302-8.
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