Mouse Anti-Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen) (APC)
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma, mammary carcinomas, ovarian tumors and prostate cancer.
Applications
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Optimal dilution determined by the researcher.
Positive Control
Tonsil tissue, A431 Cell Lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Nuclear protein preparation from the human cell line U937.50.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a cell-cycle-associated protein of 345kD and 395kD identical with the human Ki-67 antigen. Binds to a formalin-resistant epitope of the Ki-67 antigen. Reactivity is confined to the nuclei of proliferating cells and no crossreactivity with cytoplasmic antigens of epithelial occurs even after antigen retrieval. Immunohistochemistry labeling of fresh and frozen samples showed identical results with Ki-67 and Ki-S5.
