Mouse Anti-Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) (PE)
KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: Neat. Use 10ul of the optimal working dilution to label 10e6 cells in 100ul. Optimal dilutions to be determined by researcher.
Recommended Negative Controls
I1904-79D IgG1 Murine Negative Control (PE)
Source
Tissue culture supernatant
Reconstitution
Reconstitution: Reconstitute with 100ul sterile dH2O.
Storage and Stability
Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months at 4°C after receipt. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
Also Available
K1853: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) Mab Mo xHu K1853-01: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) Mab Mo xHu K1853-02: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) (Azide Free) Mab Mo xHu K1853-03: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) (Biotin) Mab Mo xHu K1853-04: Kir, Low Endotoxin (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) Mab Mo xHu K1853-05: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) (FITC) Mab Mo xHu K1853-06: Kir (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3) (PE) Mab Mo xHu
Immunogen
Human natural killer (NK) cell lysate
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R. Phycoerythrin (RPE).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family: CD158a, CD158b and P50.3. Specifically recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). Reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
