Goat Anti-LPO (Salivary Peroxidase, SPO, Lactoperoxidase, SAPX)
LPO from milk is identical to the lacrimal and salivary forms of the enzyme. LPO catalyzes the hydrogen peroxide oxidation of iodine and is used in labeling proteins with radioiodine. The antibody may facilitate studies involving LPO, including membrane analysis.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:128,000 Western Blot: 0.01-0.03ug/ml, observed in human lung lysates on ~70-75kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to sequence C-KHAKGRTRTAIRN from the internal region of Lactoperoxidase.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human Lactoperoxidase. Species Crossreactivity: bovine, mouse and rat
