Technical Data

L1219-90
Clone Type
Monoclonal
Host
Mouse
Source
Xenopus
Isotype
IgG1
Clone Number
5F312 (X167)
Grade
Affinity Purified
Applications
IC IF IHC WB
Crossreactivity
Bo Hu Mo Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Lamin
Lamin-B1, LMNB1, LMN2, LMNB
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (frozen): 1:10 Immunocytochemistry/Immunofluorescence: 1:10 Optimal dilution determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Nuclear pore complex-lamina fraction of Xenopus laevis (XLKE-A6 cells).
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes xenopus Lamin. Recognizes the karyoskeleton, i.e. the intermediate filament equivalent of the nucleus. Species Crossreactivity: bovine, mouse, rat, kangarro and trout

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Krohne, G. and Benavente R.: The Nuclear Lamins. A Multigene Family of Proteins in Evolution and Differentiation. Exp. Cell Res. 162, 1-10 (1986)|Franke, W.W.: Nuclear Lamins and Cytoplasmic Intermediate Filament Proteins: A Growing Multigene Family. Cell 48, 3-4 (1987) |Höger, T.H., Grund, C., Franke, W.W., Krohne G.: Immunolocalization of Lamins in the thick nuclear lamina of human synovial cells. Europ J Cell Biol 54, 150-156 (1991)|Höger TH, Zatloukal K, Waizenegger I, and Krohne G: Characterization of a second highly conserved B-type lamin present in cells previously through to contain only a single B-type lamin. Chromosoma 99, 379-390 (1990)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net