Technical Data

L1220-11B
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Isotype
IgM
Clone Number
R27
Grade
Supernatant
Applications
IF IHC WB
Crossreactivity
Bo Hu Mo Rt Xe Ze
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Lamin A, C
Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot: neat, with incubation for 1-2 hours at RT. Immunofluorescence: neat, with incubation for 1 hour at RT. Immunohistochemistry: frozen sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Nuclear pore complex-lamina fraction of bovine liver.
Form
Supplied as a liquid, 0.09% sodium azide.
Purity
Culture supernatant
Specificity
Decorates the lamina, i.e. the nucleoplasmic layer coating the nuclear envelope. Polypeptide(s) Reacting: Lamin isotypes A and C (Mr 60-75kD); meiotic lamin C2. Reactivity on Cultured Cell Lines (tested so far): RV-SMC (rat vascular smooth muscle cells). Species Crossreactivity: Xenopus laevis LA, Human LA, C Bovine LA, C Mouse LA, C Rat LA, C Trout LA Zebrafish LA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Krohne G. and Benavente R.: The Nuclear Lamins. A multigene family of proteins in evolution and differentiation. Exp Cell Res 162, 1- 10 (1986)|Franke WW: Nuclear lamins and cytoplasmic intermediate filament proteins: A growing multigene family. Cell 48, 3-4 (1987)|Höger TH, Zatloukal K, Waizenegger I, and Krohne G: Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin. Chromosoma 99, 379-390 (1990)|Höger TH, Grund C, Franke WW, Krohne G: Immunolocalization of lamins in the thick nuclear lamina of human synovial cells. Eur J Cell Biol 54, 150-156 (1991)|Alsheimer M, von Glasenapp E, Hock R, Benavente R: Architecture of the nuclear periphery of rat pachytene spermatocytes: Distribution of nuclear envelope proteins in relation to synaptonemal complex attachment sites. Mol Biol Cell 10, 1235-1245 (1999)|Von Glasenapp E, Benavente R: Fate of meiotic lamin C2 in rat spermatocytes cultured in the presence of okadaic acid. Chromosoma 109, 117-122 (2000)|Burke B and Stewart CL: Life at the edge: the nuclear envelope and human disease. Nature Mol Cell Biol 3, 575-585 (2002)
USBio References
No references available
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