Rabbit Anti-Large Proteoglycan, GAG alpha Domain (Versican, Versican Core Protein, Large Fibroblast Proteoglycan, Chondroitin Sulfate Proteoglycan Core Protein 2, Chondroitin Sulfate Proteoglycan 2, PG-M, Vcan, Cspg2)
Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, Versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ~1000kD, of which the core contributes 400kD from the 2409 amino acids, and the 12-15 covalently attached chondroitin sulfate chains make up the rest.
Applications
Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (FFPE): 10ug/ml. Tissues must be treated with Chondroitinase before staining (0.5U/ml in 100mM Tris-HCL, pH 7.3 for 30 minutes at RT) prior to blocking and staining. Western Blot: 0.5ug/ml. Optimal dilutions to be determined by the researcher.
Positive Control (IHC)
Surrounding cartilage, neural tube brain
Positive Control (WB)
Newborn mouse brain
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GST fusion protein containing aa535-598 of mouse versican.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by affinity chromatography
Specificity
Recognizes mouse versican GAG alpha domain.
