Rabbit Anti-Lck, phosphorylated (Tyr505) (Lymphocyte-specific Tyrosine Kinase)
Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Tyr-505 is located near the carboxyl terminus of Lck and, when phosphorylated, associates intramolecularly with the SH2 domain in the amino-terminal half of the protein. This helps stabilize Lck in a conformation that, biologically, is relatively inactive. In the absence of phosphorylation at Tyr-505, intramolecular binding of the carboxyl terminus to the SH2 domain does not occur, and Lck exhibits increased activity in vivo.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Full length untagged recombinant human Lck protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to Lck that contains tyrosine 505|(P06239). Species sequence homology: human and mouse.
Form
Supplied as a liquid in PBS, pH 7.4, 1mg/ml BSA (IgG free, protease free), 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Lck when phosphorylated at Tyr505 Species sequence homology: mouse Lck [pY505] (100%), chicken Lck [pY505] (91.7%), Hck [pY522] (75%), and Lyn [pY508] (83.3%).
