Technical Data

L1566-09
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
LIME-10
Grade
Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Lck Interacting Molecule (Lck-interacting Molecule, LIME, LIME1, Lck Interacting Transmembrane Adaptor 1, Lck-interacting Transmembrane Adapter 1, Lck-interacting Membrane Protein, LP8067)

Lck Interacting Molecule is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. Lck Interacting Molecule becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-Lck Interacting Molecule associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of Lck Interacting Molecule in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Applications
Suitable for use in Western Blot. Other applications tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
COOH-terminal peptide corresponding to residues 281-296 of the human Lck Interacting Molecule (KLH)
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified
Specificity
Recognizes the human Lck Interacting Molecule.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Brdickova N, Brdicka T, Angelisova P, Horvath O, Spicka J, Hilgert I, Paces J Simeoni L, Kliche S, Merten C, Schraven B, Horejsi V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17; 198(10):1453-62.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net