Mouse Anti-LRRTM3 (Leucine-rich Repeat Transmembrane Neuronal Protein 3, UNQ803/PRO1693)
Human LRRTM3 (leucine-rich repeat transmembrane neuronal 3) is a 63kD (predicted) type I transmembrane glycoprotein that is one of four LRRTM family members within the LRR protein superfamily (1). The LRRTM3 cDNA encodes 581aa, including a 389aa extracellular region that shares 98% aa identity with mouse and rat LRRTM3. Isoform 2 lacks cytoplasmic aa514-581. LRRTMs in mouse show distinct expression patterns during formation of the central nervous system (CNS) and expression persists in adult brain. LRRTM3 promotes processing of amyloid precursor protein by BACE1, and may be mutated in late-onset Alzheimer’s disease.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa31-419 from human LRRTM3 expressed in NSO cells (Q86VH5).
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human LRRTM3. Species sequence homology: Recombinant mouse LRRTM3. No cross-reactivity with recombinant human LRRTM1, 2, and 4.
