Sheep Anti-LRRN1 (Leucine-rich Repeats Neuronal Protein 1, Neuronal Leucine-rich Repeat Protein 1, NLRR-1, LRCH4, KIAA1497, Nbla10449, UNQ693/PRO1338)
LRRN1 is a 95kD member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716aa in length. It is a type I transmembrane protein that contains a 606aa extracellular region aa26-631 and a short 64aa cytoplasmic tail. The extracellular region shows eleven LRRs aa94-384 that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa26-631, human LRRN1 shares 96% aa sequence identity with mouse LRRN1.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant corresponding to aa26-631 from human LRRN1 expressed in NSO cell line.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LRRN1. Species sequence homology: Recombinant human NLRR-3 (5%).
