Goat Anti-LSP1 (Lymphocyte-specific Protein 1, 47kD Actin-binding Protein, 52kD Phosphoprotein, pp52, Lymphocyte-specific Antigen WP34, WP34)
Leukocyte-specific protein (LSP; also known as Lymphocyte-specific protein1, SP34 and pp52) is a 52kd filamentous-actin-binding intracellular phosphoprotein. LSP is localized to the internal surface of the plasma membrane, cytoplasm, and actin filaments. LSP expressed in various eukaryotic cell lines such as the melanoma cell line A7 but not in other hematopoietic lineages. Studies showed that LSP play a key role in anti-IgM-induced apoptosis of the B-cell lymphoma line as well as cytoskeleton-driven regulation. LSP contains a high-affinity F-actin binding site, which recently is identified in the basic C-terminal half.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-ASTKSRWETGEVQAQ, from human LSP1, at the internal region of the protein (NP_002330.1, NP_001013271.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LSP1. Species sequence homology: Bovine and canine.
