Chicken Anti-L1/ORF2 (LINE-1 retrotansposable element ORF2 protein, ORF2p)
The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.
Applications
Suitable for use in ELISA, Western Blot and Dot Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1:10,000 Western Blot: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptides corresponding to regions within the endonuclease domain of human L1/ORF2 (UniProt Accession #O00370) conjugated to KLH. Species Sequence Homology: chimpanzee; 100%, macaque, fruit fly, bovine, canine, opossum, rat; 69-88%
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Specificity
Recognizes human L1/ORF2.
