Technical Data

L2525-25B
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
RR433-8
Grade
Affinity Purified
Applications
E N WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Lipopolysaccharide Binding Protein (LBP, LPSBP)
LBP

LPS binding protein (LBP) is an approximately 60kD acute phase protein that is produced by hepatocytes. This protein strongly binds to LPS and has been shown to play an important role in the handling of LPS by the host. A number of functions of LBP have been reported. First, LBP transfers LPS to the LPS receptor CD14 on mononuclear phagocytes, leading to an 100-1,000-fold increased sensitivity of the cells to LPS. Furthermore, LBP can enhance the response of CD14 negative cells by acceleration of LPS binding to soluble CD14, a complex that stimulates these cells. Next, LBP transfers LPS into High Density Lipoprotein (HDL), which effectively neutralizes its biological potency. LBP was demonstrated to protect mice from septic shock caused by LPS or gram negative bacteria.

Applications
Suitable for use in Neutralization, Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1:10 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse natural and recombinant LBP. The antibody is a type II antibody blocking the LPS binding to CD14.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Le Roy, D et al; Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP complexes to CD14. J Immunol 1999, 162: 7454 2. Le Roy, D et al; Critical role of lipopolysaccharide-binding protein and CD14 in immune response against gramnegative bacteria. J Immunol 2001, 169: 2759
USBio References
No references available
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