Technical Data

L2601-02B
Clone Type
Monoclonal
Host
Mouse
Source
Bovine
Isotype
IgG2b
Clone Number
5F213
Grade
Affinity Purified
Applications
FC IF WB
Crossreactivity
Bo Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Lipoprotein Receptor, Low Density (LDLR, LDL Receptor)
Applications
Suitable for use in Western Blot, Flow Cytometry and Immunofluorescence microscopy. Other applications not tested.
Recommended Dilutions
Immunofluorescence microscopy: 1:10-1:50. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified bovine adrenal cortex LDL receptor.
Form
Supplied as a lyophilized powder with PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by affinity chromatography.
Specificity
Recognizes bovine LDL Receptor. Recognizes an epitope in the region of repeat #1 of the ligand binding region. Detects the 160kD polypeptide in human normal fibroblasts and in bovine adrenal gland. Addition of 15nM antibody results in inhibition of half-maximal LDL-binding. Species Crossreactivity: human. Does not react with rat, mouse, hamster (chinese hamster ovary cells), canine or rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Beisiegel U, Schneider WJ, Goldstein JL, Anderson RGW, Brown MS: Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J Biol Chem 256, 11923-11931 (1981). 2. Beisiegel U, Schneider WJ, Brown MS, Goldstein JL. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J Biol Chem 257, 13150-13156 (1982). 3. Schmitz G, Brüning T, Kovacs E, Barlage S. Fluorescence flow cytometry of human leukocytes in the detection of LDL receptor defects in the differential diagnosis of hypercholesterolemia. Arteriosclerosis & Thrombosis 13, 1053-1065 (1993)|4. Virgolini I, Li S-R, yang Q, Koller E, Sperr WR, Leimer M, Angelberger P, Nimpf J, Schneider W, Valent P. Characterization of L and VLDL binding sites on human basophils and mast cells. Arteriosclerosis, Thrombosis & Vascular Biology 15, 17-26 (1995).
USBio References
No references available
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