Technical Data

L3180-01J
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Isotype
IgG
Clone Number
11C1043(D61D1)
Grade
Purified
Applications
IF IP WB
Crossreactivity
Hu
Gene ID
79444
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Livin (Baculoviral IAP Repeat Containing Protein 7, BIRC7, Kidney Inhibitor of Apoptosis Protein, KIAP, Melanoma Inhibitor of Apoptosis Protein, MLIAP, ML-IAP, RING Finger Protein 50, RNF50, UNQ5800/PRO19607/PRO21344)

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70aa BIR (baculovirus inhibitor repeat) domain (1,2). Human members of the family include c-IAP1, c-IAP2, XIAP, survivin, livin and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7 and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). Livin (BIRC7/ML-IAP) is a potent anti-apoptotic IAP family member containing a single BIR domain and RING finger motif that is highly expressed in human melanomas and absent in normal tissues (5,7). It is localized in the nucleus and diffusely in the cytoplasm (5). The livin gene encodes two splicing variants, a 298aa isoform (a) and a 280aa form (b), with different biological activity to various apoptotic stimuli (8). In addition to directly inhibiting caspase activity, livin can promote the degradation of the pro-apoptotic protein Smac through the ubiquitin-proteasome pathway (9). Its preferential expression in tumor cells, along with several studies showing that downregulation of livin can promote apoptosis, has led to studies analyzing the use of livin in cancer therapy (10).

Applications
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Immunofluorescence (IF-IC): 1:100. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Ala195 of human livin.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human livin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1) Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52. (2) Deveraux, Q.L. et al. (1998) EMBO J 17, 2215-23. (3) Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33. (4) Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803. (5) Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.|(6) Deveraux, Q.L. et al. (1997) Nature 388, 300-4. (7) Vucic, D. et al. (2000) Curr Biol 10, 1359-66.|(8) Ashhab, Y. et al. (2001) FEBS Lett 495, 56-60. (9) Ma, L. et al. (2006) Cell Death Differ 13, 2079-88.|(10) Liu, B. et al. (2007) Cancer Lett 250, 168-76.
USBio References
No references available
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