L7715-05X
Clone Type
MonoclonalHost
RatSource
MouseIsotype
IgG2a,kClone Number
D7Grade
PurifiedApplications
FC IHC IP WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rat Anti-Ly-6A/E (Ly6A/E, Lymphocyte Antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Ly6a, Ly6, Ly-6A.2, Ly-6E.1, Stem Cell Antigen 1, Sca1, SCA-1, T-cell-activating Protein, TAP, Lymphocyte Activation Protein 6) (Azide Free)
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E+ lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation. Studies with this antibody suggest that Ly-6A/E may be involved in B and T lymphocyte responses, and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot, Immunohistochemistry and In vitro lymphocyte activation. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen sections Flow Cytometry: 0.5ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
IL-2-dependent mouse T-cell line CTL-L
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added. Low Endotoxin.
Specificity
Recognizes Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Ortega, G., P.E. Korty, E.M. Shevach, and T.R. Malek. 1986. J. Immunol. 137:3240-3246.2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207. 3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224. 4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663. 5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921. 7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107. 8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936. 9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204. 10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722. 11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414. 12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406. 13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.USBio References
No references available