Rat Anti-Ly-6C (Ly6C, Lymphocyte Antigen 6C2, Ly6c2, Ly-6C2, AA682074, AA959465) (HRP)
Ly-6C is a 14kD differentiation antigen, which is expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and and T-lymphocytes. Clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chi monocytes are recruited to acute peripheral inflammation and develop into Ly-6C+ exudate macrophages, whereas more mature Ly-6C-/lo monocytes are precursors for tissue macrophages and dendritic cells in steady state.
Applications
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Balb/c macrophage precursor cell hybrids
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Specificity
Recognizes mouse Ly6C The LY6C1 gene encodes a similar protein with ~95% sequence homology to LY6C2.
