Technical Data

L8010-03A-HRP
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
HRP
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Rabbit Anti-Lymphotactin (LPTN, LTN, ATAC, C Motif Chemokine 1, Chemokine (C Motif) Ligand 1, Cytokine SCM-1, Lymphotaxin, SCM1, SCM-1, SCM-1-alpha, SCM-1a, Small-inducible Cytokine C1, SCYC1, XC Chemokine Ligand 1, XCL1) (HRP)

Lymphotactin, otherwise known as XCL1, is the only member of the C-chemokine family identified so far. Unlike other members of the chemokine superfamily, Lymphotactin uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation.

Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.
Applications
Suitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 1.7-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human lymphotactin
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human lymphotactin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kennedy, J. et al. (1995) Molecular cloning and functional characterization of human Lymphotactin. J. Immunol. 155: 203-209.|2. Hedrick, J.A. et al. (1997) Lymphotactin is produced by NK cells and attracts both NK cells and T cells in vivo. J. Immunol. 158: 1533-1540.
USBio References
No references available
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