Rabbit Anti-Lymphotactin (LPTN, LTN, ATAC, C Motif Chemokine 1, Chemokine (C Motif) Ligand 1, Cytokine SCM-1, Lymphotaxin, SCM1, SCM-1, SCM-1-alpha, SCM-1a, Small-inducible Cytokine C1, SCYC1, XC Chemokine Ligand 1, XCL1) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Lymphotactin, otherwise known as XCL1, is the only member of the C-chemokine family identified so far. Unlike other members of the chemokine superfamily, Lymphotactin uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation.
Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.
Applications
Suitable for use in FLISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 1.7-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human lymphotactin
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human lymphotactin.
