L9189-65
Clone Type
PolyclonalHost
RabbitSource
RatIsotype
IgGGrade
Affinity PurifiedApplications
E IC IF WBCrossreactivity
Hu Mo RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Lysophospholipase D (LysoPLD)
Lysophosphatidic acid (LPA) is an extracellular signaling lipid that evokes multiple biological functions including induction of platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and chemotaxis. Lysophospholipase D (LysoPLD) was first discovered in 1999 as the enzyme responsible for generating LPA from lysophosphatidylcholine (LPC). It was later revealed to be identical to an autocrine motility factor, autotaxin (ATX), which plays a role in tumor progression and metastasis. LysoPLD/ATX mRNA is widely expressed with highest levels found in brain, ovary, lung, intestine, and testis. Rat LysoPLD is composed of 885 amino acids with an estimated molecular weight of 101kD. The protein is reported to be heavily glycosylated and thus its apparent size on SDS-PAGE may be run as high as 125kD. Useful positive controls include cerebrospinal fluid, mouse ascites or seminal plasma.
Applications
Suitable for use in Western Blot, ELISA and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:200 Immunofluorescence (IC): 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa573-588, KNKLEELNKRLHTKGS from rat Lysophospholipase D (LysoPLD).|Species Sequence Homology: mouse; 100% (16/16aa), human and chimpanzee; 93.75% (15/16aa).
Form
Supplied as a liquid in TBS, pH 7.4, 1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat LysoPLD. Species Crossreactivity: human and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Ishii, I., et al., Annu. Rev. Biochem. 73: 321-354 (2004). 2. Tokumura, A., et al., J. Biol. Chem. 277(42): 39,436-39,442 (2002). 3. Umezu-Goto, M., et al., J. Cell Biol. 158: 227-233 (2005). 4. Nam, S.W., et al., Oncogene 19: 241-247 (2000). 5. Sun, Y., et al., J. Biochem. 380: 749-755 (2004). 6. van Leeuwen, F.N., et al., Biochem. Soc. 31: 1209-1212 (2003). 7. Murata, J., et al., J. Biol. Chem. 269(48): 30,479-30,484 (1994).USBio References
No references available