Rabbit Anti-Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA)
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). A protein recently found to be involved in autophagy, LAMP-2, is a highly glycosylated protein associated with the lysosome. LAMP-1 shares much homology to LAMP-2 and is thought to have overlapping functions. Mice lacking LAMP-1 had very minor defects compared to those deficient in LAMP-2 expression. However, the loss of both proteins resulted in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.
Applications
Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunofluorescence: 20ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
EL4 cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 15aa from near the center of human LAMP-1. Epitope: located within aa230-280
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human LAMP-1. Species Crossreactivity: Mouse and rat. Predicted to not cross-react with LAMP-2
