L9190-09A
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
FITCIsotype
IgG1,kClone Number
5K76 (H4A3)Grade
PurifiedApplications
FC IHCCrossreactivity
Hu MkShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-Lysosome Associated Membrane Protein 1 (LAMP 1, CD107a, Igp120, IgpA) (FITC)
Lysosomal-associated membrane protein 1 (LAMP-1), also known as CD107a, is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107a is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets, tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium (1-7).
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 10e6 cells Immunohistochemistry: Acetone-fixed frozen tissue sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human adherent peripheral blood cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity
Recognizes human LAMP1. Species Crossreactivity: African Green monkey, baboon, chimpanzee, Pigtailed macaque, Cynomolgus monkey and Rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD107a, 107b Section, Academic Press, New York, p. 389. 3. Sawada, R., et al., J. Biol. Chem. 268: 9014 (1993). 4. Febbraio, M., & Silverstein, R.L., J. Biol. Chem. 265: 18,531 (1990). 5. Chen, J.W., et al., J. Biol. Chem. 263: 8754 (1988). 6. Sawada, R., et al., J. Biol. Chem. 268: 12,675 (1993). 7. Sawada, R., et al., J. Biol. Chem. 269: 1425 (1994).USBio References
No references available