Rat Anti-Lysosome Associated Membrane Protein 2 (LAMP 2, Igp2, Igp110, CD107b, LAMP2C, LAMPB, Lysosome Associated Membrane Glycoprotein 2 Precursor, MAC3)
Murine CD107b, also known as MAC-3 and LAMP-2, is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between 92-120kD. Studies suggest that CD107b may be involved in leukocyte adhesion.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required, Immunohistochemistry: Paraffin and frozen sections Optimal dilutions to be determined by the researcher.
Hybridoma
P3-NS1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Glycoproteins purified from mouse peritoneal macrophage membranes.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes murine Lysosome Associated Membrane Protein 2.
