Mouse Anti-Lysozyme (LYZ, LZM, 1,4-beta-N-acetylmuramidase C, Lysozyme C) (PE)
EC=3.2.1.17
Lysozyme is an enzyme that is present in mucosal secretions such as saliva and tears and is involved with non-specific organism defense. It functions by hydrolyzing 1,2-beta linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues such as those found in the walls of various Gram-positive bacteria.
Applications
Suitable for use in FLISA, Flow Cytometry, and Immunohistochemistry (paraffin). Other applications not tested.
Recommended Dilution
Immunohistochemistry (paraffin): requires antigen retrieval using heat treatment prior to staining of paraffin sections. Flow Cytometry: 1:10, 10ul labels 10e6 cells in 100ul, membrane permeabilization is required for this application Optimal dilutions to be determined by the researcher.
Positive Control Tissue
Human Tonsil
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Native, from urine.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Lysozyme.
