Rat Anti-Macrophage Colony Stimulator Factor (M-CSF, CSF-1, MCSF, Lanimostim, Macrophage colony-stimulating factor 1 precursor) (PE)
M-CSF R (macrophage colony stimulating factor receptor, c-fms) is a 165kD, 977 amino acid (aa) type I membrane glycoprotein. It is a member of the type III subfamily of receptor tyrosine kinases that contain five extracellular immunoglobulin-like domains and a cytosolic split kinase domain. M-CSF receptor is expressed primarily on monocytes, macrophages, dendritic cells, and stem cells, and regulates lineage decisions and maturation of these cells. The mouse M-CSF R extracellular domain shares > 99% aa identity with rat and 60-63% aa identity with human, dog, cat and bovine M-CSF R. It can be released by TACE/ADAM17 cleavage.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 1x10e6 cells. Validated using J774 mouse reticulum cell sarcoma macrophage cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Ala20-Ser511 from mouse M-CSF R, expressed in NS0 cells.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes mouse M-CSF R. In direct ELISA, no crossreactivity with recombinant human M-CSF R, recombinant mouse PDGF Rb or recombinant mouse FLT-3 Ligand is observed.
