Technical Data

M1201-57A
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MDC (Macrophage-derived Chemokine)

MDC (macrophage-derived chemokine) is a 69-amino acid CC chemokine with a NH2-terminal sequence of 24 residues as a signal sequence. It was originally cloned from human monocyte-derived macrophages. Recombinant MDC attracts monocyte-derived dendritic cells, activated lymphocytes and natural killer cells. MDC has anti-HIV-1 activity. Expression of MDC mRNA in macrophages is enhanced by LPS, IL-1b and TNFa. MDC is a functional ligand for CCR4.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-expressed mouse MDC
Form
Supplied as a lyophilized powder, 0.09% sodium azide. Reconsitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes both mouse and rat MDC.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Godiska, R. et al (1997) Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells. J Exp Med 185:1595-1604 2. Pal, R. et al. (1997) Inhibition of HIV-1 infection by the beta-chemokine MDC. Science 278:695-698 3. Rodenburg, R.J. (1998) Expression of macrophage-derived chemokine (MDC) mRNA in macrophages is enhanced by interleukin-1beta, tumor necrosis factor alpha, and lipopolysaccharide. J Leukoc Biol 63:606-611 4. Imai, T. et al. (1998) Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4. J biol Chem 273: 1764-1768
USBio References
No references available
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