Mouse Anti-Macrophage Inflammatory Protein-1 alpha (MIP-1a) (Liquid)
Macrophage inflammatory protein 1a (MIP-1a), also known as CCR3, belongs to the MIP-1 CC chemokine family. The cytokine is mainly produced by macrophages after stimulation by bacterial endotoxins. In acute inflammatory stage, MIP-1a plays an important role in the recruitment and activation of granulocytes. MIP-1a also stimulates the production of pro- inflammatory cytokines, including IL-1, IL-6 and TNF-a. In normal status, MIP- 1a plays a role in promoting homoeostasis.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0-AG4.1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified recombinant human MIP-1a.
Form
Supplied as a liquid in PBS, pH 7.2
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes recombinant human MIP-1a. Does not show any crossreaction with other human cytokines or growth factors tested such as M-CSF, GM-CSF, IL-7, IL-8, IL-16, IL-1B, bFGF, TGF-B, EGF, TNF-a and EPO.
