Technical Data

M1202-34A
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
IP N WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MIP-1b (Macrophage Inflammatory Protein-1 b)

MIP-1b (macrophage inflammatory protein-1 b) is a 69-amino acid CC chemokine with a molecular weight of 7.8kD. MIP-1b induces migration of monocytes/macropages, and activate CD4+ cells, but not NK cells and eosinophils.

Applications
Suitable for use in Western Blot and Neutralization. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Titer (Western Blot)
1:2,000
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-expressed recombinant fragment corresponding to amino acids 1-69 of mature rat MIP-1b.
Form
Supplied as a lyophilized powder in 0.09% sodium azide. Reconstitute with 500ul sterile ddH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes both mouse and rat natural MIP-1b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Sherry, B., et al. (1988) Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1-beta. J. Exp. Med. 168:2251-2259 2. Minano, F.J. et al. (1996) Macrophage inflammatory protein-1beta (MIP-1beta) produced endogenously in brain during E. coli fever in rats. Eur. J. Neurosci. 8:424-428 3. Wolpe, S.D. and Cerari, A. (1989) Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J 3:2565-2573 4. Wells, T.N.C. (1996) The molecular basis of selectivity between CC and CXC chemokines: the possibility of chemokine antagonists as anti-inflammatory agents. Ann N Y Acad Sci 796:245-256.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net