Rabbit Anti-Macrophage Inflammatory Protein 3 alpha (MIP-3-alpha, MIP3A, MIP-3a, Beta Chemokine Exodus-1, CC Chemokine LARC, C-C Motif Chemokine 20, Chemokine (C-C Motif) Ligand 20, CCL20, CKb4, Liver and Activation-regulated Chemokine, LARC, Small-inducible Cytokine A20, SCYA20, ST38) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
MIP-3 alpha acts as a chemoattractant for memory T lymphocytes, immature dendritic cells and B lymphocytes, being the only CC chemokine known to bind to the G-protein linked 7-transmembrane receptor CCR6. Further properties of MIP-3 alpha include antimicrobial activity, inhibition of the in vitro proliferation of bone marrow immature myeloid progenitors and the stimulation of the growth and migration of pancreatic carcinoma cells.
Applications
Suitable for use in FLISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 15-18ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human MIP-3 alpha.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human MIP-3 alpha, (Macrophage Inflammatory Protein 3 alpha), otherwise known as CCL20, a 70 amino acid CC chemokine predominantly expressed by the liver, lymph nodes.
