M1252-ML550
Clone Type
MonoclonalHost
RatSource
MouseConjugate
MaxLight™550Isotype
IgG2bClone Number
2Q1735 (MOMA-2)Grade
Affinity PurifiedApplications
FC IHCCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rat Anti-Macrophages, Monocytes (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in Flow Cytometry (membrane permeabilization is recommended) and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Mouse lymph node stroma
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes an intracellular antigen of mouse macrophages and monocytes. Reacts strongly with macrophages in lymphoid organs such as tingible body macrophages in T-cell dependent areas. Reacts on all mouse strains tested.
