Technical Data

M1275D
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2b,k
Clone Number
MECA-367
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Madcam1 (Mucosal Addressin Cell Adhesion Molecule 1, MAdCAM-1, mMAdCAM-1)

MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin a4b7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. This antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of this monoclonal antibody is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.

Applications
Suitable for use in Flow Cytometry. Though not tested, Immunohistochemistry (frozen), Immunoprecipitation and Western Blot have been reported in literature (ref 1,5 and 6). Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Endothelial cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse MAdCAM-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Streeter PR, et al. 1988. Nature 331:41.|2. Briskin MJ, et al. 1993. Nature 363:461.|3. Berlin C, et al. 1993. Cell 74:185.|4. Bargatze RF, et al. 1995. Immunity 3:99.|5. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.|6. Savinov AY, et al. 2003. J. Exp. Med. 197:643.|7. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.|8. Hindley JP, et al. 2012. Cancer Res. 72:5473
USBio References
No references available
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