Technical Data

M1750-12
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC
Crossreactivity
Hu
Accession #
NP_005358
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-MAGEA12, NT (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12)

MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human MAGEA12 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human MAGEA12.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Mallon, A.M., et al., Genome Res. 10(6):758-775 (2000). 2.Rogner, U.C., et al., Genomics 29(3):725-731 (1995). 3.Ding, M., et al., Biochem. Biophys. Res. Commun. 202(1):549-555 (1994). 4.De Smet, C., et al., Immunogenetics 39(2):121-129 (1994). 5.De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net