Technical Data

M2250-01C
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3E7
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Mannose Binding Protein (MBP, Mannan Binding Lectin, MBL)

Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q–independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.

Applications
Suitable for use in Western Blot, Immunoassays, Immunohistochemistry, and Flow Cytometry. Can be used for blocking the complement-activating capacity of human MBL. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Western Blot: 1:50 Immunohistochemistry (frozen): 1:50, Tissue sections embedded in ornithine carbamoyltransferase compound were frozen in acetone dry ice and immersed in Tris-buffered saline (TBS) three times for 5 minutes. Treatment in 0.5% hydrogen peroxide in methanol solution has been done to quench endogenous peroxidase activity Optimal dilutions to be determined by the researcher.
Negative Control
Nonimmune normal serum or TBS
Endotoxin
<24EU/mg
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Serum isolated human RaRF
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Mannose Binding Lectin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Muto, S et al; Biological activities of human mannose-binding lectin bound to two different ligand sugar structures, Lewis A and Lewis B antigens and high-mannose type oligosaccharides. Biochimica et Biophysica Acta 2001, 1527: 39 2. Matsushita, M et al; Human mannose-binding protein is identical to a component of Ra-reactive factor. Biochem Biophys Res Commun 1992, 183: 645 3. Hisano, S et al; Mesangial IgA2 deposits and lectin pathway-mediated complement activation in IgA glomerulonephritis. Am J Kidney Dis 2001, 38: 1082 4. Vries de, B et al; The mannose-binding lectin-pathway is involved in complement activation in the course of renal ischemia-reperfusion injury. Am J Pathol 2004, 165: 1677 5. Nauta, A et al; Mannose-binding lectin engagement with late apoptotic and necrotic cells. Eur J Immunol 2003, 33 : 2853 6. Nauta, A et al ; Opsonization with C1q and Mannose-binding lectin targets apoptotic cells to dendritic cells. J Immunol 2004, 173: 3044
USBio References
No references available
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