Technical Data

M2250-01D
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
9G226 (3E7)
Grade
Affinity Purified
Applications
FC FLISA IHC
Crossreactivity
Hu
Accession #
NP_000233
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Mannose Binding Protein (Mannan Binding Protein, MBP, Mannan Binding Lectin, MBL) (FITC)

Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q–independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species.

Applications
Suitable for use in Western Blot, FLISA, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50 Western Blot: 1:50 Immunohistochemistry (Frozen): 1:50. Not suitable for staining paraffin embedded sections Optimal dilutions to be determined by the researcher.
Negative Control
Nonimmune normal serum or TBS
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Serum isolated human RaRF
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Mannose Binding Lectin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Muto, S et al;Biochimica et Biophysica Acta 2001, 1527: 39 2. Matsushita, M et al; Biochem Biophys Res Commun 1992, 183: 645 3. Hisano, S et al; Am J Kidney Dis 2001, 38: 1082 4. Vries de, B et al; Am J Pathol 2004, 165: 1677 5. Nauta, A et al; Eur J Immunol 2003, 33 : 2853 6. Nauta, A et al ; J Immunol 2004, 173: 3044
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net