Rabbit Anti-MAPKAP1 (Mitogen-activated Protein Kinase 2-associated Protein 1, Mitogen-activated Protein Kinase Associated Protein 1, MIP1, Stress-activated Map Kinase-interacting Protein 1, SAPK-interacting Protein 1, mSIN1, SIN1, Target of Rapamycin Complex 2 Subunit MAPKAP1, TORC2 Subunit MAPKAP1)
JC310, MGC2745, SIN1b, SIN1g
SAPK interacting protein 1, a stress-activated protein kinase, is an evolutionarily conserved component of the eukaryotic SAPK pathway belonging to the SIN1 family. There are multiple isoforms with one being a 522aa protein with 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. SAPK interacting protein 1 forms a complex with inactive and nonphosphorylated MEKK2 and in the process regulates its dimerization and activation. It is also known to maintain CRTC2 complex integrity and regulates Akt phosphorylation and cell signaling. Recent reports suggest that it acts as scaffold molecule in the regulation of signaling by JNK and takes part in RAS signal pathway. It expresses in all tissues examined, with highest levels in skeletal muscle and heart.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human, mouse and rat skeletal muscle lysate.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa100-150 of human SAPK interacting protein 1.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human SAPK interacting protein 1/Mip1. Species Crossreactivity: bovine, canine, mouse, rat.
