Rabbit Anti-MAP Kinase 6 (Mitogen-activated Protein Kinase 6, MAPK 6, MAPK6, PRKM6, Extracellular Signal-regulated Kinase 3, ERK3, ERK-3, HsT17250, MAP Kinase Isoform p97, p97-MAPK, p97MAPK)
EC=2.7.11.24
ERK3, also known as MAPK6 or p97 MAPK, is almost 50% identical to ERK1/2 at the kinase domain located in its amino-terminal region (1). However, ERK3 is distinguished from other MAP kinases in that it lacks the conserved TXY motif in its activation loop, possessing instead an SEG motif (1,2). Phosphorylation at Ser189 in the SEG motif has been reported (2,3). With limited information about its upstream kinases and downstream substrates, the significance of this phosphorylation remains to be elucidated 3,4). ERK3 is an inherently unstable protein, rapidly degraded through amino-terminal ubiquitination and proteasome degradation (3,5). A site-specific cleavage, depending on a short stretch of acidic residues of ERK3, might regulate its translocation from the Golgi/ERGIC to the nucleus during the cell cycle (6). Accumulating evidence suggests that ERK3 is involved in cell differentiation (1,3,6).
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding Leu410 of human ERK3 (KLH-coupled).
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Detects endogenous levels of total human ERK3 protein. The antibody does not cross-react with other other ERK family members. Species Crossreactivity: mouse, monkey and rat
