M2414-ML650
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™650Isotype
IgG1,kClone Number
2Q1505 (B7)Grade
Affinity PurifiedApplications
IHCCrossreactivity
HuAccession #
NP_001827Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Mast Cell Chymase (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in Immunohistochemistry. Not suitable for use in formaldehyde fixed tissue. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): Heat induced epitope retrieval with citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified Mast Cell Chymase from human skin.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human mast cell chymase (27kD).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Tadashi, T., et al., J. Hepatology 33: 961-966 (2000). 2. Buckley, M., et al., J. Pathol. 186: 67-74 (1998). 3. Kleinjan, A., et al., Allergy 51: 614-620 (1998). 4. Clin. Exp. Allergy 20: 53-58 (1990). 5. J. Allergy Clin. Immunol. 86: 34-39 (1990). 6. J. Immunol. 145: 2652-2661 (1990). 7. Lab. Invest. 63: 581-585 (1990). 8. Lab. Invest. 58: 682-691 (1989). 9. Lab. Invest. 60: 147-157 (1989). 10. J. Histochem. Cytochem. 37: 1509-1515 (1989). 11. PNAS USA 86: 10,039-10,043 (1989). 12. J. Immunol. 138: 2184-2189 (1987). 13. J. Immunol. 138: 4381-4386 (1987). 14. J. Immunol. 138: 2611-2615 (1987). 15. PNAS USA 83: 4464-4458 (1986).USBio References
No references available